Journal: Cell Transplantation

Article Title: Histone Methyltransferase KMT2D Regulates H3K4 Methylation and is Involved in the Pathogenesis of Ovarian Cancer

doi: 10.1177/09636897211027521

Figure Lengend Snippet: (A) ESR1 expression in OV stage II–IV was higher than stage I and Fig. 1B low survival probability related to high ESR1 level from TCGA ( P = 0.022) (B) Immunohistochemical staining images (up) and scores (down) of KMT2D, ESR1 and H3K4me in tumor tissues of phase I ( n = 38), phase II ( n = 58), phase III ( n = 60) and phase IV ( n = 42) OV patients and adjacent tissues (including tumor samples and adjacent tissues.). (phase I vs phase II P < 0.05, phase II vs phase III P < 0.001, phase III vs phase IV N.S. , two-sided unpaired t -test). (C) The protein expression of KMT2D, ESR1, PTGS2, STAT3, VEGFR2 in tumor tissues of phase I ( n = 38), phase II ( n = 58), phase III ( n = 60) and phase IV ( n = 42) OV patients and adjacent tissues (including 198 tumor samples and 133 adjacent tissues). (phase I vs phase II P < 0.05, phase II vs phase III P < 0.001, phase III vs phase IV N.S. , two-sided unpaired t -test). (D) The binding of H3K4me and ESR1 was demonstrated using CHIP analysis and the relative expression of ESR1 was calculated via normalized to the Input group. Scale bar: 50 μm. * P < 0.05 vs adjacent tissues, ** P < 0.01 vs adjacent tissues, # P < 0.05 vs tumor (phase I), ## P < 0.01 vs tumor (phase I).

Article Snippet: The adenovirus vector for KMT2D interference, the plasmid for point mutation at H3K4 site, as well as the activator and inhibitor of KMT2D were constructed by GenePharma Co., Ltd (Shanghai, China), which were subsequently transfected into SKOV3 and HO-8910 cell lines for 48 h, respectively.

Techniques: Expressing, Immunohistochemical staining, Staining, Binding Assay

Journal: Cell Transplantation

Article Title: Histone Methyltransferase KMT2D Regulates H3K4 Methylation and is Involved in the Pathogenesis of Ovarian Cancer

doi: 10.1177/09636897211027521

Figure Lengend Snippet: (A) Expression of KMT2D, ESR1, H3K4me in IOSE80 and SKOV3 cell lines were determined using immunofluorescence and located by DAPI staining. (B) Expression of KMT2D, ESR1, FOXL2, PTGS2, and VEGFR2 detected by western blotting. The relative expression was calculated via normalization to the expression of actin. Scale bar: 20 μm. * P < 0.05 vs IOSE80, ** P < 0.01 vs IOSE80, # P < 0.05 vs model control group, ## P < 0.01 vs model control group.

Article Snippet: The adenovirus vector for KMT2D interference, the plasmid for point mutation at H3K4 site, as well as the activator and inhibitor of KMT2D were constructed by GenePharma Co., Ltd (Shanghai, China), which were subsequently transfected into SKOV3 and HO-8910 cell lines for 48 h, respectively.

Techniques: Expressing, Immunofluorescence, Staining, Western Blot

Journal: Cell Transplantation

Article Title: Histone Methyltransferase KMT2D Regulates H3K4 Methylation and is Involved in the Pathogenesis of Ovarian Cancer

doi: 10.1177/09636897211027521

Figure Lengend Snippet: (A) OV tumor size in mice was measured by ruler (mm 3 ). (B) Relative mRNA expression of ESR1, ELF3, and STAT3 determined using qRT-PCR and calculated via normalization to the mRNA level of GAPDH. (C) Expression of KMT2D, ESR1, H4K3me, PTGS2, and VEGFR2 detected by western blotting. The relative expression was calculated via normalization to the expression of actin. (D) The binding of H3K4me and ESR1 was demonstrated using CHIP analysis and the relative expression of ESR1 was calculated via normalized to the Input group. * P < 0.05 vs IOSE80, ** P < 0.01 vs IOSE80, # P < 0.05 vs model control group, ## P < 0.01 vs model control group.

Article Snippet: The adenovirus vector for KMT2D interference, the plasmid for point mutation at H3K4 site, as well as the activator and inhibitor of KMT2D were constructed by GenePharma Co., Ltd (Shanghai, China), which were subsequently transfected into SKOV3 and HO-8910 cell lines for 48 h, respectively.

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Binding Assay

Journal: Scientific Reports

Article Title: Rotavirus Calcium Dysregulation Manifests as Dynamic Calcium Signaling in the Cytoplasm and Endoplasmic Reticulum

doi: 10.1038/s41598-019-46856-8

Figure Lengend Snippet: RV-induced dynamic Ca 2+ signaling is related to virus dose. ( A) Immunofluorescence images of mock or SA114F-infected MA104-GCaMP5G cells that were inoculated with increasing MOI (0.1, 1, or 10). RV antigen (red) is detected at ~8 hpi with anti-RV polyclonal antisera and nuclei are stained with DAPI (blue). (B , C) Epifluorescence images of MA104-GCaMP5G cells mock or infected with recombinant SA11-mRuby reporter virus with increasing MOIs (0.1, 1, 10). Images were captured at 7 HPI (B) or 10 HPI (C) . (D) Representative single-cell traces of relative GCaMP5G fluorescence (F/F 0 ) from cells mock (black) or RV infected by SA114F with MOIs of 0.1 (purple), 1 (blue), 10 (red). (E) Representative single-cell traces of relative fluorescence (F/F 0 ) of GCaMP5G (green) and mRuby (red) from cells infected by SA11-mRuby MOI 0.1 (purple) or 1 (blue). (F , G) Number of Ca 2+ spikes (F/F 0 > 5%) from mock or RV-infected cells that were infected with SA114F (F) or SA11-mRuby (G) . Data shown as mean ± SD of 60 cells/condition. **p < 0.01.

Article Snippet: Lentivirus constructs encoding shRNA targeting SA11 gene 10 and a non-targeting scrambled shRNA negative control were generated and packaged by America Pharma Source, LLC.

Techniques: Virus, Immunofluorescence, Infection, Staining, Recombinant, Fluorescence